MegaSporeBiotic & MegaPreBiotic Study

Bacillusstrains and a prebiotic fiber blend consistently enhanced metabolic activity

This article is a summary of the study performed on MegaSporeBiotic and MegaPrebiotic.

This study was conducted to assess the effects of the daily use of Megasporebiotic a 5 spore-forming Bacillus strains probiotic in combination with a prebiotic fiber blend MegaPrebiotic on the human gastrointestinal tract.

Prebiotics and probiotics are used to improve the health of the intestinal tract.  Prebiotics are non-digestible fibers that are used by the gut bacteria. Probiotics are considered live micro-organisms.              

Prebiotics are formulated with fibers such as inulin, fructooligosaccharides (FOS), galactooligosaccharides(GOS) and xylooligosaccharides (XOS).

Probiotic strains that are most often used are Lactobacillus spp., Bifidobacterium spp., Saccharomyces boulardii and Bacillus coagulans.

The combination of Prebiotics and Probiotic supplementation has been linked to several health benefits such as improvement of atopic dermatitis(1), reduction of serum lipid profile in patients with type-2 diabetes(2), alleviation of digestive complaints in patients with gastro-intestinal disorders(3) and changes in anthropometric measurements in obese individuals(4).    

In this study Microbiome Labs provided MegaSporebiotic which contains Bacillus indicus (HU36), Bacillus subtilis (HU58), Bacillus coagulans SC-208, Bacillus licheniformis and Bacilllus clausii SC-109 spores and MegaPrebiotic, a blend of prebiotics of fructooligosaccharides from green and gold kiwi furit, xylooligosaccharides from corn cob and ga-lactooligosaccharides from cow milk. 

The daily dosage contained 2 capsules of MegaSporeBiotic and 3775 mg of MegaPrebiotic.    

The test was an in vitro simulation of the human intestinal microbial ecosystem. The setup represented different regions of the human GI tract; the stomach, small intestine and the ascending, the transverse and decending colon.  

Fecal matter was used from three different human adults. Samples for microbial community analysis were collected once a week.

The results showed that the treatment with MegaSporeBiotic and MegaPrebiotic in combination showed a consistently strong increase in butyrate levels for all donors tested.

The results also showed a significant increase in Lactobacillus spp.. This effect was not present when probiotic blend alone was used.

The study showed that these observations stress the fact that the combination of functional fibers (as in MegaPrebiotic) with probiotic strains (as in MegaSpore) could be a useful strategy in modulating the microbial community, as they might selectively stimulate different microbial groups.

Butyrate is used an energy source for the colon lining which provides health promoting properties. Those include anti-inflammatory activity, anti-cancer effects, promotion of satiety and reduction of oxidative stress(5).

FOS from gold kiwi fruit, which is one of the ingredients in MegaPrebiotic increased Faecalibacterium prausnitzii levels significantly in functionally constipated individuals. (6)  Faecalibacterium prausnitzii exerts strong anti-inflammatory activity in the intestinal environment which is mainly linked with the production of butyrate stimulation of regulatory T-cells (7) Furthermore, increased Faecalibacterium prausnitzii levels have been associated with reduction of inflammatory markers (8) and reduction of endotoxemia in obese subjects.


The combination of Megaspore, which contains five spore-forming Bacillus strains, and a prebiotic MegaPrebiotic, a blend of FOS, GOS and XOS, “consistently affected microbial activity and composition in the human gastrointestinal tract in vitro, with profound effects being observed on colonic butyrate production.

Moreover, when compared with a previous study investigating the impact of the probiotic strains on colonic functionality, the generated data suggest a synergistic effect on the gut microbiome for the synbiotic test product. Given the fact that the probiotic formulation has already been shown to impact metabolic endotoxemia in human individuals, it might be interesting to further investigate the efficacy of the synbiotic formulation in tackling metabolic disorders.”

Connect with me on Social Networks: (just scroll down a bit) Videos and Info on Gut Health & EMF protection, Gardening, & General Health Issues

The information provided on this website is not meant to substitute professional medical advice. Consult your health care provider in regard to supplementation, especially if you are on any kind of medication.

1 Farid, R., Ahanchian, H Jabbari, F., Moghiman, T. 2011.
Effect of a new symbiotic mixture on atopic dermatitis in children: 
a randomized-controlled trial. Iran. J. Pediatr. 21, 225-230.

2 Shakeri, H., Hadaegh, H., Abedi, F., Tajabadi-Ebrahimi,
M., Mazroii, N., Ghandi, Y., Asemi, Z., 2014. 
Consumption of symbiotic bread decreases triacylglycerol 
and VLDL levels while increasing HDL levels in serum from 
patients with type-2 diabetes. Lipids 49, 695-701.

3 Fujimori, S., Gudis, K., Mitsui, K., Seo, T., Yonezawa,
M., Tanaka, S., Tatsuguchi, A., Sakamoto, C., 2009. 
A randomized controlled trial on the efficacy of symbiotic versus probiotic or prebiotic treatment 
to improve the quality of life in patents with ulcerative colitis. Nutrition 
Burbank, Los Angeles County, Calif.) 25, 520-525.  Smid 2016

4 Ipar, N., Aydogdu, S.D., Yildirim, G.K., Inal, M.,
Gies, I., Vandenplas, Y., Dinleyici, E. 2015. 
Effects of symbiotic on anthropometry, lipid profile and oxidative stress in obese children. 
Beneficial Microb. 6, 775-782.

5  Hamer, H.M.,
Jonkers, D., Venema, K., Vanhoutvin, S. Troost, F.J., Brummer, R.J., 2008. 
Review article: the role of butyrate on clonic function. Aliment. Pharmacol. Ther. 27, 104-119.

6 Blatchford P., Stoklosinski, H.m Eady, S., Wallace, A.,
Butts, C., Gearry, R., Gibson, G., Ansell, J., 2017. 
Consumption of kiwifruit capsules increases Faecalibacterium prausnitzii abundance in functionally 
constipated individuals: a randomized controlled human trial. 
J. Nutr. Sci. 6e52-e52.

7 Furusawa, Y., Obata, Y., Fukuda, S., Endo, T.A.,
Nakato, G., Takahashi, D., Nakanishi, Y., Uetake, C., Kato, K., Kato, T.,Takahashi, M., Fukuda, N.N., 
Murakami, S., Miyauchi, E., ino, S., Atarashi, K.,Onawa, S., Fujimura, Y., Lockett, T., Clarke, J.M., 
Topping, D.L., Tomita, M.,Hori, S., Ohara, O., Morita, T., Koseki, H., Kikuchi, J., Honda, K., Hase, K.,
Ohno, H.,  2013;  
Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells. 
Nature 504, 446-450.

8 Furet, J.P., Kong, L.C., Tap, J., Poitou, C.,Basdevant, A., Bouillot, J.L., Mariat, D., Corthier, G., 
Dore, J., Henegar, C.,
Rizkalla, S., Clement, K., 2010. 
Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with 
metabolic and low-grade inflammation markers. 
Diabetes 59, 3049-3057.

9 Gonzalez-Sarrias, A., Romo-Vaquero, M.,Garcia-Villalba, R., Cortes-Martin, A., Selma, M.V., Espin, J.C., 
The endotoxemia marker lipopolysaccharide-binding protein is reduced in overweight-obese subjects consuming 
pomegranate extract by modulating the gut microbiota: 
a randomized clinical trial. 
Mol. Nutr. Food Res. 62, e1800160.